Volume 13, Issue 6 (Feb & Mar 2020)
payavard 2020, 13(6): 429-438 |
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Rezaei M, Mehrabian S, Amini K. The Synergistic Effect of Ketoconazole and Probiotic Bifidobacterium Bifidum on Isolated Composition of Expression of Candida Albicans Als Gene Biofilm from Oral Samples. payavard 2020; 13 (6) :429-438
URL: http://payavard.tums.ac.ir/article-1-6910-en.html
URL: http://payavard.tums.ac.ir/article-1-6910-en.html
1- Master of Science in Microbiology, Department of Biology, Faculty of Basic Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
2- Professor, Department of Microbiology, Faculty of Basic Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran ,mehrabian_s@aui.ac.ir)
3- Associate Professor, Department of Microbiology, Faculty of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
2- Professor, Department of Microbiology, Faculty of Basic Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran ,
3- Associate Professor, Department of Microbiology, Faculty of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
Abstract: (2433 Views)
Background and Aim: Candida albicans has numerous virulence factors such as the agglutinin-like sequence(ALS) genes which code the large glycoprotein family that has a role in the adherence of Candida. The present study was to observe the synergistic effect of ketoconazole and probiotic composition of Bifidobacterium bifidum on expression of C. albicans als gene biofilm isolated from oral samples.
Materials and Methods: In this cross-sectional study, 12 clinical isolates of C. albicans were collected from oral periodontal infection in patients referred to dental clinic in Kerman. The MIC and FIC values for each treatment(keto and probiotic alone and keto-probiotic composition) were obtained using micro broth dilution method. Finally, a real-time PCR test was performed to evaluate the level of ASL gene expression in the strains and the results were analyzed using the 2-ΔΔct method.
Results: The results showed that the combination of ketoconazole and bifidobacterium bifidum had synergistic effects. The results of this study showed that the effect of Ketoconazole(keto), B.bifidum(probiotic) alone and the effects of ketoconazole+Bifidobacterium(keto+pro) were 1.47, 1.61 and 1.29 times, reduced the als gene, respectively.
Conclusion: The results of this study showed that synergistic effects between ketoconazole and probiotic B. bifidum have been shown to reduce als gene expression(biofilm production). Therefore, it is recommended to administer probiotic supplementation with ketoconazole in the treatment of candidal infections.
Materials and Methods: In this cross-sectional study, 12 clinical isolates of C. albicans were collected from oral periodontal infection in patients referred to dental clinic in Kerman. The MIC and FIC values for each treatment(keto and probiotic alone and keto-probiotic composition) were obtained using micro broth dilution method. Finally, a real-time PCR test was performed to evaluate the level of ASL gene expression in the strains and the results were analyzed using the 2-ΔΔct method.
Results: The results showed that the combination of ketoconazole and bifidobacterium bifidum had synergistic effects. The results of this study showed that the effect of Ketoconazole(keto), B.bifidum(probiotic) alone and the effects of ketoconazole+Bifidobacterium(keto+pro) were 1.47, 1.61 and 1.29 times, reduced the als gene, respectively.
Conclusion: The results of this study showed that synergistic effects between ketoconazole and probiotic B. bifidum have been shown to reduce als gene expression(biofilm production). Therefore, it is recommended to administer probiotic supplementation with ketoconazole in the treatment of candidal infections.
Keywords: Candida Albicans, agglutinin like sequence(Als) Gene, Ketoconazole, Bifidobacterium Bifidum, Real-time Polymerase Chain Reaction
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