Volume 8, Issue 3 (9-2014)                   payavard 2014, 8(3): 249-257 | Back to browse issues page

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Kashani Khatib Z, Dehghanifard A, Kaviani S, Noruzinia M, Mohammadi M, Mohammad Ali F, et al . Invitro Evaluation Of The Effect Of Fetal Hemoglobin Inducer Drugs On Proliferation And Differentiation Of Erythroid Progenitors With β -Thalassemia Gene Mutation. payavard 2014; 8 (3) :249-257
URL: http://payavard.tums.ac.ir/article-1-5505-en.html
Invitro Evaluation Of The Effect Of Fetal Hemoglobin Inducer Drugs On Proliferation And Differentiation Of Erythroid Progenitors With β -Thalassemia Gene Mutation
Zahra Kashani Khatib1 , Ali Dehghanifard2 , Saeid Kaviani3 , Mehrdad Noruzinia4 , Momeneh Mohammadi5 , Fatemeh Mohammad Ali5 , Elham Roshandel5 , Sahar Mohammadi Fateh6 , Shaban Alizadeh * 7
1- Master of Sciences Student in Hematology, Hematology Department, School of Medical Sciences, Tarbiat Modares University & Students Scientific Research Center(SSRC), Tehran University of Medical Sciences, Tehran, Iran
2- Ph.D Student in Hematology, Hematology Department, School of Medical Sciences, Tarbiat Modares University and Sarem Cell Research Center (SCRC), Sarem Women’s Hospital, Tehran, Iran
3- Associate Professor, Hematology Department, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
4- Associate Professor, Medical Genetics Department, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
5- Ph.D Student in Hematology, Hematology Department, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
6- Bachelor of Sciences in Medical Laboratory, Sarem Cell Research Center (SCRC), Sarem Women’s Hospital, Tehran, Iran
7- Assistant Professor, Hematology and Transfusion Medicine Department, School of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran , alizadehs@sina.tums.ac.ir
Abstract:   (7449 Views)

 Background and Aim: Understanding the molecular mechanisms involved in the increased levels of HbF inducing drugs should be advised for effective induction. The aim of this study was to investigate the molecular effects of the drugs thalidomide and sodium butyrate considered as HbF inducer agents.

 Materials and Methods: In this experimental study, CD133+ cord blood stem cells carrying mutations of heterozygous β-thalassemia were isolated and differentiated into erythroid lineage. In order to evaluate the expression of the erythroid markers, CD71 and CD235a, was analysed. For this purpose, the RNA extracted from erythroid precursors at days 6 and 12 of erythroid differentiation and cDNA synthesized, and then the expression of these genes was performed by quantitative Real-time PCR technique.

 Results: The results of this study showed the significant effect of thalidomide on erythroid proliferation as compared to sodium butyrate and control group (P<0.05). Also, thalidomide significantly increased CD71expression and decreased CD235a expression as compared to sodium butyrate and control groups (P<0.05).

 Conclusion : Thalidomide may play its role on HbF induction by increasing the proliferation of early erythroid precursors.

Keywords: Thalidomide, Sodium Butyrate, Erythroid Precursors, CD71, CD235a
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Type of Study: Original Research | Subject: Hospital Managment
ePublished: 1399/07/23
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