Volume 6, Issue 4 (15 2012)                   payavard 2012, 6(4): 282-292 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghasemi M, Nadali F, Ostad S N, Zaker F, Rostamy S, Dargahi H. Assesment Of The Efficacy Of HESA-A On The Proliferation And Apoptosis Of Chronic Myelogenous Leukemia Cell Line(K562). payavard. 2012; 6 (4) :282-292
URL: http://payavard.tums.ac.ir/article-1-20-en.html
Abstract:   (7821 Views)

Background and Aim: Chronic myelogenous leukemia is characterized by Philadelphia (Ph) chromosome, the presence of BCR-ABL fusion gene and constitutive activation of the ABL1 tyrosine kinase. Despite an excellent result of target therapy by imatinib, some patients develop resistance to imatinib. In this study Efficacy of HESA-A on proliferation and apoptosis of K562 cell line was assessed.

Materials and Methods: In this study doubling time of K562 cell line was calculated. The cells were affected by various concentrations of HESA-A(1,2,4 and 8 mg/ml respectively). Cytotoxicity and IC50 dose of HESA-A were detected by MTT and trypan blue exclusion assay. Apoptosis was assessed by flowcytometry after 48 h cell treatment in the presence of IC50 dose.

Results: Doubling time of K562 cells was 24 hours. HESA-A reduced the number of viable K562 cells in a concentration dependent manner.IC50 dose was 3.5 mg/ml. In flowcytometry analysis of apoptosis, 19.22% of the treated cells were located in the position of the necrotic cells.

Conclusion: The results of MTT and trypan blue exclusion assay suggest that HESA-A inhibits the growth of k562 cells in a concentration dependent manner and induces necrosis in K562 cells.

Full-Text [PDF 290 kb]   (3786 Downloads)    
Type of Study: Research | Subject: Hospital Managment
ePublished: 1399/07/23

Add your comments about this article : Your username or Email:

© 2021 All Rights Reserved | Journal of Payavard Salamat

Designed & Developed by : Yektaweb