Volume 12, Issue 4 (Oct & Nov 2018)
payavard 2018, 12(4): 309-323 |
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Bahrami-Banan F, Sheikhha M H, Ghasemi N, Majdizadeh M, Haghiralsadat B F. Preparation and Study of Nano-Niosomes Containing Doxorubicin and Evaluation of its Toxicity on Acute Myeloblastic Leukemia Cell Line KG-1. payavard 2018; 12 (4) :309-323
URL: http://payavard.tums.ac.ir/article-1-6592-en.html
URL: http://payavard.tums.ac.ir/article-1-6592-en.html
Fatemeh Bahrami-Banan1 
, Mohammad Hasan Sheikhha2 , Nasrin Ghasemi2 , Mohammad Majdizadeh3 , Bibi Fatemeh Haghiralsadat * 4
, Mohammad Hasan Sheikhha2 , Nasrin Ghasemi2 , Mohammad Majdizadeh3 , Bibi Fatemeh Haghiralsadat * 4
1- Master of Science in Biotechnology, Genetics Department, Biotechnology Research Center, International Campus, Shahid Sadoughi University of Medical Science, Yazd, Iran
2- Ph.D. in Genetics, Genetics Department, Biotechnology Research Center, International Campus, Shahid Sadoughi University of Medical Science, Yazd, Iran
3- Master of Science in Cellular and Molecular Biology, Nano-Biotechnology Department, Nano-Biotech Foresight Company Biotechnology Campus, Science & Technology Park of Yazd, Yazd, Iran
4- Ph.D. in Nano-Biotechnology, Advanced Medical Sciences and Technologies Department, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , fhaghirosadat@gmail.com
2- Ph.D. in Genetics, Genetics Department, Biotechnology Research Center, International Campus, Shahid Sadoughi University of Medical Science, Yazd, Iran
3- Master of Science in Cellular and Molecular Biology, Nano-Biotechnology Department, Nano-Biotech Foresight Company Biotechnology Campus, Science & Technology Park of Yazd, Yazd, Iran
4- Ph.D. in Nano-Biotechnology, Advanced Medical Sciences and Technologies Department, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , fhaghirosadat@gmail.com
Abstract: (3310 Views)
Backgrounds and Aim: One of the effective strategies for targeting chemotherapy in the treatment of cancer is the use of lipid nano-carriers. In this study, an optimal formulation of niosomal drug containing doxorubicin has been developed to better fight cancer cells.
Material and Methods: Niosomal vesicles were prepared using phosphatidylcholine (22%), span60 (52/5%), cholesterol (22/5%) and DSPE-PEG2000 (5%) by thin-film method. Doxorubicin were loaded into the niosomes using inactive loading method. Their physico-chemical features were assayed using Zeta-Sizer, FTIR and SEM, and drug release amount was calculated at 37° C and 44° C. At the end, the toxicity of the nano drug carrier system was measured on the KG-1 cell line of the bone marrow cancer by MTT method.
Results: Niosomal vesicles containing Doxorubicin showed the size of 160.37±65.2 nm, 94.18% drug encapsulation efficiency -58.11± 1.24 mV of zeta potential and polydispersity index (PDI) of 0.234±0.02 The prepared niosomal system presented drug controlled release and FTIR investigation showed almost no interaction between nano-carrier containing drug and the drug itself. As well, morphological examination of nano-carriers using SEM microscopy revealed that they have spherical structures. Also, cellular studies showed that drug toxicity was higher in encapsulated conditions compared to non-encapsulated conditions.
Conclusion:The results of this study, meanwhile confirming the proper physicochemical characteristics of the system and being Slow-release system indicate that this nano-carrier anionic increases the toxicity of the drug for the KG-1 cell line of the bone marrow, thus, this niosomal nano-carrier can be a suitable carrier for drug delivery to cancer cells.
Material and Methods: Niosomal vesicles were prepared using phosphatidylcholine (22%), span60 (52/5%), cholesterol (22/5%) and DSPE-PEG2000 (5%) by thin-film method. Doxorubicin were loaded into the niosomes using inactive loading method. Their physico-chemical features were assayed using Zeta-Sizer, FTIR and SEM, and drug release amount was calculated at 37° C and 44° C. At the end, the toxicity of the nano drug carrier system was measured on the KG-1 cell line of the bone marrow cancer by MTT method.
Results: Niosomal vesicles containing Doxorubicin showed the size of 160.37±65.2 nm, 94.18% drug encapsulation efficiency -58.11± 1.24 mV of zeta potential and polydispersity index (PDI) of 0.234±0.02 The prepared niosomal system presented drug controlled release and FTIR investigation showed almost no interaction between nano-carrier containing drug and the drug itself. As well, morphological examination of nano-carriers using SEM microscopy revealed that they have spherical structures. Also, cellular studies showed that drug toxicity was higher in encapsulated conditions compared to non-encapsulated conditions.
Conclusion:The results of this study, meanwhile confirming the proper physicochemical characteristics of the system and being Slow-release system indicate that this nano-carrier anionic increases the toxicity of the drug for the KG-1 cell line of the bone marrow, thus, this niosomal nano-carrier can be a suitable carrier for drug delivery to cancer cells.
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