Volume 11, Issue 6 (3-2018)                   payavard 2018, 11(6): 685-693 | Back to browse issues page

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Ranjbar E, Amini K. Identification of Pyoverdine Gene in the Human and Animal in Pseudomonas aeruginosa Isolates by Multiplex-PCR Method. payavard. 2018; 11 (6) :685-693
URL: http://payavard.tums.ac.ir/article-1-6429-en.html
1- Master of Science in Microbiology, Microbiology Department, School of Basic Sciences, Islamic Azad University, Saveh Branch, Saveh, Iran
2- Assistant Professor, Microbiology Department, School of Basic Sciences, Islamic Azad University, Saveh Branch, Saveh, Iran , dr_kumarss_amini@yahoo.com
Abstract:   (386 Views)
Background and Aim: Pseudomonas aeruginosa is one of the most important factors in hospital infections, especially in patients with immune deficiency and Childhood diseases. The Virulence of bacteria are due to the presence of the Pyoverdine gene, which has many effects on the wild type of bacteria during the pathogenic pathway. Identification of different classes of PVD gene is necessary for the development of prevention and control Diseases program. In this research, the presence of PVD genes in the samples and their effect on pathogenicity was isolated and investigated.
Materials and Methods: In the present study 60 Species of P. aeruginosa was isolated from clinical samples of human and animal, after approval by diagnostic tests and differential, were studied. Finally, for every 60 Species, isolated, Multiplex PCR was performed to detect target genes. Multiplex PCR method is to be considered as the gold standard. Its results are more reliable.
Results: The results showed that the frequency of PVD gene in human isolates and livestock gene encoding the 3rd Pyoverdine was the highest frequency with 76.6% the lowest frequency is for Pyoverdine class 2 with 46%.
Conclusion: As a result, the identification of three type’s genes of PVD classes in all strains tested by Pseudomonas could help to identify human patients and livestock with Pseudomonas infection and, given the presence of the gene encoding PVDs, has a direct relation to important bacterial pathogenicity. 
Full-Text [PDF 686 kb]   (138 Downloads)    
Type of Study: Research | Subject: Laboratory Sciences
ePublished: 2018/03/12

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