Volume 8, Issue 5 (1-2015)                   payavard 2015, 8(5): 368-378 | Back to browse issues page

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Tavasoli B, Safa M, Kazemi A. Synergistic Apoptotic Effect Of Indole-3-Carbinol And Doxorubicin On Pre-B Acute Lymphoblastic Leukemia Cells. payavard 2015; 8 (5) :368-378
URL: http://payavard.tums.ac.ir/article-1-5529-en.html
1- Master of Sciences in Hematology & Blood Banking, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran
2- Assistant Professor, Hematology & Blood Banking Department, School of Allied Medicine, Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
3- Professor, Hematology & Blood Banking Department, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran , A.kazemi@iums.ac.ir
Abstract:   (7776 Views)

Background and Aim: Indole-3-carbinol (I3C), found in Brassica species vegetables, exhibits antitumor effects. It has been shown that I3C induces apoptosis in various cell types through inactivation of the nuclear factor-kappa B (NF- k B) pathway. Anthracyclines such as doxorubicin, is widely used in the treatment of hematological malignancies, induce apoptosis in tumor cells via DNA damage and activation of p53. However, NF- k B pathway that activated by anthracyclines as a part of DNA damage response can induce chemo resistance. In this study the apoptotic effect of doxorubicin in combination with NF- k B inhibitor I3C was assessed in acute lymphoblastic leukemia cells.

Materials and Methods: Human pre-B acute lymphoblastic leukemia cell line, NALM-6 cells, were preincubated with various concentrations of I3C for1 hour and then treated with 125nM doxorubicin at 37 ° C for 24 hours. Cellular DNA content assay and Annexin V-FITC staining were performed by flowcytometry for evaluation of apoptosis.

Results: DNA histogram analysis of NALM-6 cells indicates that combination of I3C with doxorubicin synergistically escalated the percentages of sub-G1 population cells (apoptotic cells) as compared to doxorubicin-only treated group. Annexin V-FITC staining showed that cotreatment of NALM-6 cells with I3C and doxorubicin increased the proportion of Annexin-V positive cells (early apoptotic cells) in comparison with the doxorubicin treated cells.

Conclusion: The results of cell culture treatments and cell death analysis by flowcytometry suggest that I3C synergistically potentiates doxorubicin-induced apoptosis in human leukemia NALM-6 cells.

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Type of Study: Original Research | Subject: Hospital Managment
ePublished: 1399/07/23

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