Volume 9, Number 3 (9-2015)                   payavard 2015, 9(3): 276-287 | Back to browse issues page


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Saberian M, Shahidi Delshad E, Naji T, Samadikuchaksaraei A. Comparing The Effect Of Lactobacillus Aacidophilus And MRS Medium On Mesenchymal Stem Cells Proliferation. payavard. 2015; 9 (3) :276-287
URL: http://payavard.tums.ac.ir/article-1-5771-en.html

1- Master of Science in Cellular and Molecular Biology, Medical Laboratory Sciences Department, School of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran
2- Master of Science in Critical Care Nursing, Critical Care Nursing Department, Rajaei Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran
3- Assistant Professor, Biology Department, Pharmaceutical Branch, Islamic Azad University, Tehran, Iran
4- Associate Professor, Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran , ali.samadi@iums.ac.ir
Abstract:   (2462 Views)

Background and Aim: The use of probiotics has been surveyed in different studies. For example, many researches have been conducted on immunity, cancer and embryonic cells proliferation. Mesenchymal stem cells (MSCs) have a rather slower growth and a more limited number of passages. In this research, the effect of supernatant of Lactobacillus acidophilus (LS) as one of the most commonly used probiotics on the proliferation of mesenchymal stem cells has been studied with the aim of increasing their proliferation for the treatment of patients in need of transplantation using mesenchymal stem cells.

Materials and Methods: The MSCs separated from rats’ bone marrow were led to bone and fat levels in the second passage, and their mesenchymal state was confirmed. The cells were then treated by the supernatant of Lactobacillus acidophilus (LS) and MRS broth medium, which have been separated in advance. The number of cells was examined by MTT test equated with the standard.

Results: A curve was drawn for the cells’ growth in different amounts and the number of cells was obtained by converting the results of MTT test to a standard curve. For statistical analyses, SPSS and ANOVA were employed.

Conclusion: The findings show the double effect of LS on the weak effect of MRS broth medium. The use of supernatant of Lactobacillus acidophilus (LS) can be a practical and economical method for increasing the proliferation of MSCs isolated from bone marrow.

Full-Text [PDF 408 kb]   (1356 Downloads)    
Type of Study: Research | Subject: Hospital Managment
Received: 2015/10/25 | Accepted: 2015/10/25 | Published: 2015/10/25

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