Volume 8, Number 4 (11-2014)                   payavard 2014, 8(4): 269-279 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sabbaghi M, Kazemi A, Hassani S, Alimoghaddam K, Ghavamzadeh A, Ghaffari S H. Evaluation Of BIBR1532 And ATRA Combination In NB4 Cell Line. payavard. 2014; 8 (4) :269-279
URL: http://payavard.tums.ac.ir/article-1-5484-en.html

1- Master of Sciences in Hematology & Blood Banking, School of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran
2- Professor, Hematology & Blood Banking Department, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran
3- Associate Professor, Hematology-Oncology and Stem cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran
4- Professor, Hematology-Oncology and Stem cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran
5- Associate Professor, Hematology-Oncology and Stem cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran , Shghaffari200@ yahoo.com
Abstract:   (6550 Views)

Background and Aim: Acute promyelocytic leukemia (APL) is a distinct type of leukemia which is caused due to a blockage in myeloid cells normal maturation. The most important therapeutic strategies include the use of ATRA and Arsenic trioxide. Although ATRA is generally well tolerated, some patients develop Retinoic acid syndrome. Some of the symptoms of this syndrome are directly or indirectly related to elevated WBC counts. This study aims to determine the effect of ATRA and BIBR1532 combination on WBC count as a factor leading to the formation of ATRA syndrome.

Materials and Methods: To investigate the effect of BIBR1532 and ATRA combination, NB4 cells were cultured in the presence of 30μ M and 1 μM densities of the drugs. To study the effect of drugs on living cells count, proliferation activity, and metabolic activity of the cells, Trypan blue, Brdu and MTT tests were used, respectively.

Results: The results of Trypan blue, MTT and Brdu suggest that the combination of ATRA and BIBR1532 is more effective than ATRA alone on the reduction of viable cell count, metabolic activity and proliferation of leukemic cells in the first five days of treatment.

Conclusion: The results suggest that the combination of ATRA and BIBR1532 is probably more effective in the treatment of APL patients. It seems that such improvement in results is more obvious especially among the patients who are at a higher risk of ATRA syndrome. 

Full-Text [PDF 413 kb]   (1260 Downloads)    
Type of Study: Research | Subject: Hospital Managment
Received: 2014/11/8 | Accepted: 2014/11/8 | Published: 2014/11/8

Add your comments about this article : Your username or email:
Write the security code in the box

Send email to the article author


© 2015 All Rights Reserved | Journal of Payavard Salamat

Designed & Developed by : Yektaweb