Volume 2, Number 1 and 2 (2 2008) | payavard 2008, 2(1 and 2): 40-46 | Back to browse issues page


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Araghi M, Alimoghadam K, Einollahi N, Chardooli B, Rahimi H, Rostami S et al . Detection of PLZF- RARa fusion in patients with Acute Promyelocytic Leukemia. payavard. 2008; 2 (1 and 2) :40-46
URL: http://payavard.tums.ac.ir/article-1-148-en.html

Abstract:   (6055 Views)

Background and Aim: Acute promyelocytic leukemia (APL) is associated with the t(1517) ,fusing promyelocytic leukemia (PML) and retinoic acid receptor-a (RARa) genes. This disease is uniquely sensitive to treatment with all-trans retinoic acid (ATRA) and highly responsive to conventional chemotherapy. The t(1117)(q23q21) abnormality associated with a PLZF-RARa rearrangement is the commonest of the alternative translocations accounting for less than 1% APL. Blasts from PLZF-RARa cases have been found to be resistant to the differentiating effects of retinoids. In this study we aimed to determine the PLZF-RARa and fusion genes in patients with APL morphology who referred to Hematology-Oncology and BMT research center, Tehran Shariaty Hospital in 2006.

Materials & Methods: Peripheral blood and/or bone marrow samples were taken from 200 patients with APL morphology and 200 patients with other subtypes of AML. The mono-nuclear cells were enriched by centrifugation over a ficoll-isopaque gradient. RNA extracted by Trizol or TriReagent and then reverse transcribed to cDNA using random primers. PCR performed using specific primers for each fusion. PCR products electerophoresed on a 2% agarose gel containing 0.05% ethidiume bromide.

Results: In 2 (1%) patients with APL-morphology the RT-PCR analysis showed PLZF-RARa fusion transcripts.

Conclusion: It can be concluded that RT-PCR is a rapid and sensitive method for detection of abnormal fusion genes in leukemia and allows the definition of a correct strategy for treatment and subsequent minimal residual disease monitoring

Full-Text [PDF 262 kb]   (968 Downloads)    
Type of Study: Research | Subject: Hospital Managment
Received: 2008/05/2 | Accepted: 2008/05/22 | Published: 2013/07/27

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