Volume 10, Issue 6 (3-2017)                   payavard 2017, 10(6): 523-529 | Back to browse issues page


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Tajedini M, Sadighi Gilani M A, Heydari T, Choobineh K, Choobineh H. Detection of Herpes Simplex Virus 1 and 2 in Semen, Blood and Urine of Idiopathic Infertile Men and Its Association with Sperm Count and Motility. payavard. 2017; 10 (6) :523-529
URL: http://payavard.tums.ac.ir/article-1-6145-en.html

1- Veterinarian, School of Veterinary Science, Shiraz University, Shiraz, Iran
2- Professor, Urology Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3- Master of Sciences Student in Developmental Biology, School of Biological Sciences, Islamic Azad University of Parand, Tehran, Iran
4- Master of Science in Biology, Helal Specialty and Subspecialty Center of Infertility (Rouyesh), Tehran, Iran
5- Assistant Professor, Medical Laboratory Sciences Department, School of Allied Medical Sciences, Zoonosis Research Center, Tehran University of Medical Sciences, Tehran, Iran , hchobineh@tums.ac.ir
Abstract:   (1826 Views)

Background and Aim: Infertility is a major problem of modern medicine as it affects almost 20% of reproductive-aged couples. The cause of this problem is attributed to the male partner in nearly 40%-50% of these cases. The role of herpes simplex virus (HSV) in male infertility has been investigated using the sensitive methods. The aim of this study was to determine the prevalence of HSV-1, 2 DNA in the semen, blood and urine of idiopathic infertile men and its association with altered semen parameters.
Materials and Methods: A total of 150 semen, blood and urine samples from infertile men were collected in the Shariati hospital, Tehran (2012-2014). Sample analysis and diagnostic PCR using specific primers was performed for detection of HSV-1, 2 DNA in the specimens.
Results: Analysis showed six groups of infertile men, including HSV-1, 2 positive and negative groups in semen, blood and urine samples. HSV-1, 2 DNA was detected in 38 (18.66%) semens, (6.66%) 10 in blood and 2 (1.33%) in urine. Only HSV-1, 2 positive samples of semen had abnormal semen parameters.
Conclusion: Using a powerful molecular method, we detected a high prevalence of HSV-1, 2 DNA in the semen of asymptomatic infertile patients. Although HSV-1, 2 infections were not associated with motility and morphology defects of the sperms, it was related with decreased sperm count in the semen fluid. In addition there was not a significant role for detection of HSV-1, 2 DNA in blood and urine samples of infertile men and abnormal semen parameters.

Full-Text [PDF 423 kb]   (635 Downloads)    
Type of Study: Research | Subject: Laboratory Sciences
Received: 2017/03/14 | Accepted: 2017/03/14 | Published: 2017/03/14

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