Volume 9, Issue 3 (9-2015)
payavard 2015, 9(3): 249-255 |
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Telefian C, Kalantar E, Soltan Dallal M M. Isolation And Identification Of Burkholderia Cepacia From Respiratory Secretions In Cyctic Fibrosis Patients. payavard 2015; 9 (3) :249-255
URL: http://payavard.tums.ac.ir/article-1-5739-en.html
URL: http://payavard.tums.ac.ir/article-1-5739-en.html
1- Master of Sciences in Microbiology, School of Basic Sciences, Islamic Azad University, Science and Research of Kurdistan Branch, Sanandaj, Iran
2- Associate Professor, Microbiology and Immunology Department, School of Medicine, Alborz University of Medical Sciences, Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran
3- Professor, Microbiology Section, Pathobiology Department, School of Public Health, Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran , Soltanda@sina.tums.ac.ir
2- Associate Professor, Microbiology and Immunology Department, School of Medicine, Alborz University of Medical Sciences, Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran
3- Professor, Microbiology Section, Pathobiology Department, School of Public Health, Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran , Soltanda@sina.tums.ac.ir
Abstract: (10290 Views)
Background and Aim: Cystic fibrosis is a common hereditary and autosomal
disorder. One of the factors in cystic fibrosis is Burkholderia cepacia which can be
transmitted through the sharing of admitted patients with hospitalized patients.
Purpose of this study, was isolation and identification of Burkholderia cepacia from
respiratory secretions from Masih Daneshvari Hospital cystic fibrosis patient.
Materials and Methods: In this study, during 8 months from 2011 till
2012, 100 cases of pulmonary secretions were collected in Masih Daneshvari
hospital patients. The specimens were cultured on both MacConkey agar and
Burkholderia cepacia selective agar (BCSA) medium. After an incubation of 48
hours at 35°C, suspected colonies were isolated and by using of phenotypic and
biochemical tests like TSI agar, simmons citrate medium, indol and motility, MRVP,
oxidation - fermentation (OF), arginine- ornithine - lysine decarboxylase test,
Dnase test, and polymyxin resistance were identified.
Results: Using BCSA medium and biochemical tests led to isolation and
identification of five Burkholderia cepacia strains from 100 respiratory samples.
However, using Mac Conkey no Burkholderia cepacia was detected.
Conclusion: Our results clearly indicate the use of BCSA medium as a specific
medium for isolation of Burkholderia cepacia in cystic fibrosis patients. So,
presence of 5% Burkholderia cepacia in cystic fibrosis infections suggests
detecting this bacterium, in a specific and sensitive culture medium.
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